Peripherin Antibody - BSA Free

Novus Biologicals | Catalog # NB300-137

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all Peripherin Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human peripherin expressed in and purified from E. coli.

Localization

Neuronal Marker

Marker

Un-myelinated Neurons Marker

Specificity

Specifically recognizes peripherin, showing no cross reactivity with other Class III intermediate filament proteins.

Clonality

Polyclonal

Host

Rabbit

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Peripherin Antibody - BSA Free

Western Blot: Peripherin Antibody [NB300-137]

Western Blot: Peripherin Antibody [NB300-137]

Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.
Immunocytochemistry/ Immunofluorescence: Peripherin Antibody [NB300-137]

Immunocytochemistry/ Immunofluorescence: Peripherin Antibody [NB300-137]

Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Analysis of the widely used rat pheochromocytoma PC12 cell line stained with rabbit polyclonal Periphren antibody, dilution 1:2000 (Green). Hoechst staining of nuclear DNA (Blue). Peripherin, one of the Class III family of intermediate filament subunit proteins, is a major component of the PC12 cell forming a perinuclear cap, with some filaments visible in the cytoplasm.
Western Blot: Peripherin Antibody [NB300-137]

Western Blot: Peripherin Antibody [NB300-137]

Western Blot: Peripherin Antibody [NB300-137] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule.
Immunocytochemistry/ Immunofluorescence: Peripherin Antibody [NB300-137]

Immunocytochemistry/ Immunofluorescence: Peripherin Antibody [NB300-137]

Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-137 antibody to peripherin (green) and chicken polyclonal antibody to phosphorylated NF-H (red channel). A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain NF-H and not peripherin. The blue channel reveals the localization of DNA.

Applications for Peripherin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000

Immunohistochemistry

1:2000

Western Blot

1:1000-1:2000
Application Notes
This Peripherin antibody can be used for Immunocytochemistry/Immunofluorescence, Western blotting, and Immunohisochemistry. In WB a band can be seen at ~57 kDa. Use in IHC-P and IHC-Fr reported in scientific literature (PMID: 16325747 and 24277578 respectively).

Reviewed Applications

Read 1 review rated 5 using NB300-137 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Supplied as serum

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peripherin

Peripherin (PRPH) is a 57kD type III neuronal intermediate filament (NIF) protein which is predominantly expressed in neurons of PNS and in certain CNS neurons specifically from brain stem/spinal cord which generally project toward peripheral structures. Inside developing neurons, PRPH is expressed abundantly in the growth cones and is required for axonal outgrowth as well as maintenance or regeneration. In mature neurons, together with other NIFs, PRPH constitutes a shape-maintaining IF network. Besides its crucial role in development of nervous system, PRPH can also be highly relevant to pathogenesis of neurodegenerative disorder. PRPH expression is induced in CNS neurons (wherein it is not expressed normally) upon brain injury, and factors such as LIF, IL-6 etc and PRPH molecules form aggregates in neurons. Synaptic plasticity is markedly altered in PRPH transgenic mice (Per mice) and up-regulation of PRPH expression is implicated in some pathophysiological conditions, such as cerebral ischemia and seizure, and some cognitive disorders. In ALS patients, PRPH together with neuronal IFs are detected in the majority of abnormal IF inclusion bodies in mature or aging motor neurons.

Alternate Names

Neurofilament 4, neurofilament 4 (57kD), peripherin, PRPH1NEF4

Entrez Gene IDs

5630 (Human)

Gene Symbol

PRPH

Additional Peripherin Products

Product Documents for Peripherin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Peripherin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Peripherin Antibody - BSA Free

Customer Reviews for Peripherin Antibody - BSA Free (1)

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  • Name: Mao Yang
    Application: Immunohistochemistry
    Sample Tested: Spinal cord
    Species: Mouse
    Verified Customer | Posted 08/17/2010

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