Peripherin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-05423

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Format

BSA Free
View all Peripherin Primary Antibodies »

Product Specifications

Immunogen

Recombinant rat peripherin expressed in and purified from E. coli

Marker

Un-myelinated Neurons Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Peripherin Antibody - BSA Free

Western Blot: Peripherin Antibody [NBP1-05423]

Western Blot: Peripherin Antibody [NBP1-05423]

Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.
Immunocytochemistry/ Immunofluorescence: Peripherin Antibody [NBP1-05423]

Immunocytochemistry/ Immunofluorescence: Peripherin Antibody [NBP1-05423]

Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - Analysis of mixed fibroblast and PC12 pheochromocytoma cell culture stained with chicken pAb to peripherin, NBP1-05423, dilution 1:5,000 in green, and costained with rabbit pAb to vimentin, RPCA-Vim, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. NBP1-05423 antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.
Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423]

Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423]

Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (red) in nerve bundles in the human larynx. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Peripherin Antibody [NBP1-05423]

Immunocytochemistry/ Immunofluorescence: Peripherin Antibody [NBP1-05423]

Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - shows rat mixed neuron/glial cultures stained with NBP1-05423, our chicken polyclonal antibody to peripherin (green channel) and rabbit antibody to the neurofilament subunit alpha-internexin NBP1-05207 (green channel). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and alpha-internexin, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain.
Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423]

Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423]

Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (green) in the mouse brainstem including the spinal trigeminal tract (right side of image) and arching fiber bundle to the solitary nuclear tract (center). Nissl Counterstain. Image from verified customer review.

Applications for Peripherin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000-1:5000

Immunohistochemistry

1:20000

Western Blot

1:20000
Application Notes
This Peripherin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~57 kDa. IHC-Fr reported in verified customer review.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-05423 in the following applications:

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as a concentrated total IgY preparation from egg yolk, dialyzed against PBS with added preservative.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peripherin

Peripherin (PRPH) is a 57kD type III neuronal intermediate filament (NIF) protein which is predominantly expressed in neurons of PNS and in certain CNS neurons specifically from brain stem/spinal cord which generally project toward peripheral structures. Inside developing neurons, PRPH is expressed abundantly in the growth cones and is required for axonal outgrowth as well as maintenance or regeneration. In mature neurons, together with other NIFs, PRPH constitutes a shape-maintaining IF network. Besides its crucial role in development of nervous system, PRPH can also be highly relevant to pathogenesis of neurodegenerative disorder. PRPH expression is induced in CNS neurons (wherein it is not expressed normally) upon brain injury, and factors such as LIF, IL-6 etc and PRPH molecules form aggregates in neurons. Synaptic plasticity is markedly altered in PRPH transgenic mice (Per mice) and up-regulation of PRPH expression is implicated in some pathophysiological conditions, such as cerebral ischemia and seizure, and some cognitive disorders. In ALS patients, PRPH together with neuronal IFs are detected in the majority of abnormal IF inclusion bodies in mature or aging motor neurons.

Alternate Names

Neurofilament 4, neurofilament 4 (57kD), peripherin, PRPH1NEF4

Entrez Gene IDs

24688 (Rat)

Gene Symbol

PRPH

Additional Peripherin Products

Product Documents for Peripherin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Peripherin Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Peripherin Antibody - BSA Free

Customer Reviews for Peripherin Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
0%
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1 Stars
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Customer Images

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  • Peripherin Antibody
    Name: Thomas Finger
    Application: Immunohistochemistry-Frozen
    Sample Tested: larynx
    Species: Human
    Verified Customer | Posted 09/17/2018
    Peripherin staining (red) in nerve bundles in the human larynx.
    Fixation 4% buffered paraformaldehyde.
    Peripherin Antibody - BSA Free NBP1-05423
  • Peripherin Antibody
    Name: Thomas Finger
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 09/17/2018
    Peripherin staining (green) in the mouse brainstem including the spinal trigeminal tract (right side of image) and arching fiber bundle to the nuc. solitary tract (center). Nissl Counterstain.
    Tested on both human and mouse tissues. Works well for staining of small diameter nerve fibers in peripheral tissues. Less effective in staining central terminals of sensory axons (mouse) but still effective (see image).
    Peripherin Antibody - BSA Free NBP1-05423

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Protocols

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