Recombinant Human Peroxiredoxin 1 His Protein

Novus Biologicals | Catalog # NBC1-18543

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE
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Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-199 of Human Peroxiredoxin 1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSGNAKIGH PAPNFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWVNT PKKQGGLGPM NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITVNDLPVG RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Peroxiredoxin 1 His Protein

SDS-PAGE: Recombinant Human Peroxiredoxin 1 His Protein [NBC1-18543]

SDS-PAGE: Recombinant Human Peroxiredoxin 1 His Protein [NBC1-18543]

SDS-Page: Recombinant Human Peroxiredoxin 1 His Protein [NBC1-18543] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Functional: Recombinant Human Peroxiredoxin 1 His Protein [NBC1-18543]

Functional: Recombinant Human Peroxiredoxin 1 His Protein [NBC1-18543]

Functional: Recombinant Human Peroxiredoxin 1 His Protein [NBC1-18543] - The remaining peroxide content was determined by measuring the red-colored ferrithiocyanate complex formed as result of reaction at 475nm. delta H2O2 means the discrepancy of peroxide amount before and after reaction. This test was performed triplicate.

Formulation, Preparation, and Storage

NBC1-18543
Formulation 20 mM Tris-HCl buffer (pH 7.5), 20% Glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 1

PRDX1 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein is also known to be important to protect red blood cells against reactive oxygen species and in tumor prevention. Recombinant human PRDX1, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

NKEF-A, PRDX1, PRX1, TDPX2

Gene Symbol

PRDX1

Additional Peroxiredoxin 1 Products

Product Documents for Recombinant Human Peroxiredoxin 1 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Peroxiredoxin 1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human Peroxiredoxin 1 His Protein

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