PF 543 hydrochloride

Tocris Bioscience | Catalog # 5754

Potent and selective SphK1 inhibitor
Tocris Bioscience
Discontinued Product
5754 has been discontinued. View all Sphingosine Kinase Inhibitors products.

Key Product Details

Description

Potent and selective SphK1 inhibitor
View all Sphingosine Kinase Inhibitors »

Product Description

PF 543 hydrochloride is a potent and selective SphK1 inhibitor (IC50 = 2 nM; Ki = 3.6 nM). Exhibits >100-fold selectivity for Sphk1 over Sphk2. Also exhibits >5,000 fold selectivity over S1P1-5 receptors and 48 protein and lipid kinases. Inhibits growth and metastasis of several types of cancer cell including ovarian, colorectal and breast cancers, and suppresses HCT-116 tumor xenograft growth in mice. Antifibrotic and anti-inflammatory in a range of in vivo disease models. Also reduces sickling, hemolysis and inflammation in a transgenic mouse model of sickle cell disease.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Product Specifications for PF 543 hydrochloride

Molecular Weight

502.07

Formula

C27H31NO4S.HCl

Storage

Desiccate at RT

Purity

≥98% (HPLC)

Chemical Name

(2R)-1-[[(4-[[3-Methyl-5-[(phenylsulfonyl)methyl]phenoxy]methyl]phenyl]methyl]-2-pyrrolidinemethanol hydrochloride

CAS Number

1706522-79-3

PubChem ID

121230770

InChI Key

WNKWAZFYPZMDGJ-VQIWEWKSSA-N

SMILES

CC1=CC(OCC2=CC=C(CN3CCC[C@@H]3CO)C=C2)=CC(CS(C4=CC=CC=C4)(=O)=O)=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 5.02 10 with gentle warming
DMSO 50.21 100

Preparing Stock Solutions for PF 543 hydrochloride

The following data is based on the product molecular weight 502.07.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.99 mL 9.96 mL 19.92 mL
5 mM 0.40 mL 1.99 mL 3.98 mL
10 mM 0.20 mL 1.00 mL 1.99 mL
50 mM 0.04 mL 0.20 mL 0.40 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

Product Documents for PF 543 hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PF 543 hydrochloride

For research use only

Related Research Areas

Bioactive Lipids and Receptors

Citations for PF 543 hydrochloride

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