Recombinant Human PGK2 His Protein

Novus Biologicals | Catalog # NBP2-52287

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE
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Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-417 of Human PGK2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

46.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PGK2 His Protein

SDS-PAGE: Recombinant Human PGK2 His Protein [NBP2-52287]

SDS-PAGE: Recombinant Human PGK2 His Protein [NBP2-52287]

SDS-Page: PGK2 Recombinant Protein [NBP2-52287] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-52287
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT, 0.1 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGK2

The PGK2 gene encodes a testis-specific form of phosphoglycerate kinase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. See also PGK1 (MIM 311800), which is ubiquitously expressed in all somatic tissues and maps to chromosome Xq13.[supplied by OMIM]

Alternate Names

dJ417L20.2, EC 2.7.2.3, PGK-2, PGKB, PGKPS, phosphoglycerate kinase 2, phosphoglycerate kinase autosomal pseudogene, Phosphoglycerate kinase, testis specific

Gene Symbol

PGK2

Additional PGK2 Products

Product Documents for Recombinant Human PGK2 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human PGK2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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