PHLPP2 Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP2-13757PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Antibody Competition
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Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human PHLPP2.

Source: E. coli

Amino Acid Sequence: DLHLVLCTVETPASEICAGEGRESLYLQLHGDLVRRLEPTERPLQIVYDYLSRLGFDDPVRIQEEATNPDLGCMIRFYG

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-13757.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP2-13757PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: PHLPP2

PHLPP2 contains 21 LRR (leucine rich) repeats, 1 PH domain and 1 PP2C like domain. It is a protein phosphatase that specifically mediates dephosphorylation of AKT1 Ser 473, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro and antiapoptotic genes. Dephosphorylation of Ser 473 of AKT1 triggers apoptosis and decrease cell proliferation. PHLPP2 also controls the phosphorylation of AKT3. It binds 2 manganese ions per subunit. There are 3 named isoforms produced by alternative splicing.

Alternate Names

EC 3.1.3.16, KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase-like, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPPLPHLPP-like

Gene Symbol

PHLPP2

Additional PHLPP2 Products

Product Documents for PHLPP2 Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for PHLPP2 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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