Phorbol 12,13-dibutyrate

Tocris Bioscience | Catalog # 4153

Protein kinase C activator
Tocris Bioscience

Key Product Details

Description

Protein kinase C activator

Alternative Names

PDBu
View all Protein Kinase C Activators »

Product Description

Phorbol 12,13-dibutyrate is a protein kinase C activator. Induces contraction of vascular smooth muscle and inhibits MLC phosphatase (MLCP) in vascular smooth muscle. Activity does not alter intracellular Ca2+ concentration. Also inhibits the activity of Na+,K+ ATPase in OK cells. Used in protocol (see below) to generate pancreatic β cells from hPSCs.

For more information about how Phorbol 12,13-dibutyrate may be used, see our protocol: Generation of β cells from hPSCs

Product Specifications for Phorbol 12,13-dibutyrate

Molecular Weight

504.61

Formula

C28H40O8

Storage

Store at -20°C

Purity

≥97% (HPLC)

Chemical Name

(1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-1a,1b,4,4a,5,7a,7b,8,9,9a-Decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9,9a-diyl butanoic acid ester

CAS Number

37558-16-0

PubChem ID

37783

InChI Key

BQJRUJTZSGYBEZ-YVQNUNKESA-N

SMILES

OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 25.23 50

Preparing Stock Solutions for Phorbol 12,13-dibutyrate

The following data is based on the product molecular weight 504.61.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.96 mL 19.82 mL 39.63 mL
2.5 mM 0.79 mL 3.96 mL 7.93 mL
5 mM 0.40 mL 1.98 mL 3.96 mL
25 mM 0.08 mL 0.40 mL 0.79 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 26 publications citing the usage of this product.

Product Documents for Phorbol 12,13-dibutyrate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Phorbol 12,13-dibutyrate

For research use only

Related Research Areas

Diabetes
Intracellular Kinases

Citations for Phorbol 12,13-dibutyrate

Customer Reviews for Phorbol 12,13-dibutyrate (1)

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  • PDB reduces saxagliptin-induced APD prolongation in cardiomyocytes
    Name: Chintan Koyani
    Species: Guinea Pig
    Assay Type: In Vitro
    Cell Line/Tissue: ventricular cardiomyocytes
    Verified Customer | Posted 10/22/2018
    Guinea pig ventricular cardiomyocytes were patched and superfused with Saxagliptin (2 µM, 5 min) followed by Phorbol 12,13-dibutyrate (PDB, 10 nM, 5 min) and action potentials were stimulated at a 1 Hz frequency. Action potential duration (APD) were analysed at 20%, 50% and 90% repolarization (shown in Image). PDB reduced saxagliptin-induced APD prolongation.
    Phorbol 12,13-dibutyrate 4153

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