Phospho-Synaptotagmin‑1 (T202) Antibody

  
  • Species Reactivity
    Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Zebrafish
  • Specificity
    Rat Synaptotagmin phosphorylated at T202
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding the phospho-T202 of Synaptotagmin-1
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Phospho-Synaptotagmin-1 (T202) by Western Blot. Western Blot of rat cortex lysate showing specific immunolabeling of the ~60 - 62 kDa Synaptotagmin phosphorylated at T202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase; lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to anti-Synaptotagmin (T202). The immunolabeling is completely eliminated by treatment with lambda PPase.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: Synaptotagmin-1
Synaptotagmins are integral membrane proteins of synaptic vesicles. Synaptotagmin-1 is a glycoprotein containing two C2 domains related to protein kinase C and sites for calcium-dependent binding of acidic phospholipids. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local calcium-dependent buckling of the plasma membrane. Synaptotagmin-2 is a single-pass, type Ia/III (no signal sequence) transmembrane (TM) glycoprotein. Synaptotagmin-2 is an integral component of neurotransmitter-containing synaptic vesicles that detects action potential-induced increases in presynaptic cytosolic calcium. Increased ionic calcium binds to synaptotagmin II at two sites (C2a and C2b) on its cytoplasmic tail. The first site also binds phospholipid, while the second site binds syntaxin. This promotes vesicle membrane fusion with the presynaptic plasma membrane, resulting in neurotransmitter release.

Synaptotagmins undergo three types of posttranslational modification that may affect function. N-linked glycosylation and/or O-linked glycosylation are likely necessary for recycling (internalization) of vesicle membrane after neurotransmitter release. Fatty acylation/palmitoylation of synaptotagmin may be necessary for proper cycling. Finally, synaptoagmin phosphorylation within the C2a site regulates calcium-binding, while phosphorylation in the C2b site may regulate calcium and syntaxin interaction.
  • Entrez Gene IDs:
    6857 (Human); 20979 (Mouse); 25716 (Rat)
  • Alternate Names:
    DKFZp781D2042; P65; SVP65; synaptotagmin Isynaptotagmin 1; Synaptotagmin1; Synaptotagmin-1; SYT; SYT1; sytI
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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