Phospho-Synaptotagmin‑1 (T202) Antibody Summary
Complete Your Research
Complete Your Experiment
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Phospho-Synaptotagmin-1 (T202) by Western Blot. Western Blot of rat cortex lysate showing specific immunolabeling of the ~60 - 62 kDa Synaptotagmin phosphorylated at T202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase; lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to anti-Synaptotagmin (T202). The immunolabeling is completely eliminated by treatment with lambda PPase.
Preparation and Storage
Synaptotagmins are integral membrane proteins
of synaptic vesicles. Synaptotagmin-1 is a glycoprotein containing two
C2 domains related to protein kinase C and sites for calcium-dependent
binding of acidic phospholipids. Synaptotagmin-1 participates in the
process of vesicular trafficking and exocytosis by inducing local
calcium-dependent buckling of the plasma membrane. Synaptotagmin-2 is a
single-pass, type Ia/III (no signal sequence) transmembrane (TM)
glycoprotein. Synaptotagmin-2 is an integral component of
neurotransmitter-containing synaptic vesicles that detects action
potential-induced increases in presynaptic cytosolic calcium. Increased
ionic calcium binds to synaptotagmin II at two sites (C2a and C2b) on
its cytoplasmic tail. The first site also binds phospholipid, while the
second site binds syntaxin. This promotes vesicle membrane fusion with
the presynaptic plasma membrane, resulting in neurotransmitter release.
Synaptotagmins undergo three types of posttranslational modification that may affect function. N-linked glycosylation and/or O-linked glycosylation are likely necessary for recycling (internalization) of vesicle membrane after neurotransmitter release. Fatty acylation/palmitoylation of synaptotagmin may be necessary for proper cycling. Finally, synaptoagmin phosphorylation within the C2a site regulates calcium-binding, while phosphorylation in the C2b site may regulate calcium and syntaxin interaction.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Phospho-Synaptotagmin‑1 (T202) Antibody
There are currently no reviews for this product. Be the first to review Phospho-Synaptotagmin‑1 (T202) Antibody and earn rewards!
Have you used Phospho-Synaptotagmin‑1 (T202) Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image