Phospho-Tryptophan Hydroxylase 1/TPH‑1 (S58) Antibody

  
  • Species Reactivity
    Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit, Xenopus, Zebrafish
  • Specificity
    This antibody is specific for the ~55 kDa TPH phosphorylated at S58 in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding the phospho-S58 of Tryptophan Hydroxylase 1/TPH-1
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Phospho-Tryptophan Hydroxylase (S58) by Western Blot. Western blot of recombinant rabbit tryptophan hydroxylase in a crude bacterial lysate incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.
Background: Tryptophan Hydroxylase 1/TPH-1
Tryptophan hydroxylase (TPH) is a 51 kDa, 444 amino acid (aa) enzyme that belongs to the pterin-dependent, aromatic amino-acid hydroxylase family. It is found in peripheral tissues and pineal tissues, and catalyzes the rate-limiting step in serotonin generation from L‑tryptophan. The molecule contains two domains, an N-terminal regulatory domain (aa 1-110) and a C-terminal catalytic domain (aa 111-444) that contains an iron atom. TPH is constitutively active but unstable. Phosphorylation by PKA at S58 stabilizes the enzyme and increases its activity by 25%. This action is complemented by 14-3-3 proteins that physically interact with phosphorylated TPH to increase both its stability and activity by another 15% to 45% of baseline. Binding of the acidic carboxyterminus of 14-3-3 with the regulatory region of TPH also blocks phosphatase activity directed towards TPH, prolonging its activity. One splice variant of TPH is known. It occurs at the extreme C‑terminus where there is a 29 aa substitution for the last seven amino acids. This may generate a more stable enzyme.
  • References:
    1. Boularand, S. et al. (1990) Nucleic Acids Res. 18:4257.
    2. Wang, G-A. et al. (1998) J. Neurochem. 71:1769.
    3. Kumer, S.C. et al. (1997) J. Neurochem. 69:1738.
    4. Wang, L. et al. (2002) Biochemistry 41:12569.
    5. Banik, U. et al. (1997) J. Biol. Chem. 272:26219.
  • Entrez Gene IDs:
    7166 (Human); 21990 (Mouse); 24848 (Rat)
  • Alternate Names:
    EC 1.14.16; EC 1.14.16.4; indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; TPH; TPH-1; TPRH; TRPH; tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1; Tryptophan Hydroxylase 1/TPH-1
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