Q: Is this protein active?

A: Unfortunately, our protein NBP2-23326 has not yet been tested in an activity assay. So we unfortunately do not have any data that shows this protein is biologically active. It has only been used a positive control in SDS-Page gels.

Recombinant Human PHOSPHO1 His Protein

Novus Biologicals | Catalog # NBP2-23326

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Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all PHOSPHO1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-267 of Human PHOSPHO1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKS C

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

32.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PHOSPHO1 His Protein

SDS-PAGE: Recombinant Human PHOSPHO1 His Protein [NBP2-23326]

SDS-Page: PHOSPHO1 Protein [NBP2-23326]

Formulation, Preparation, and Storage

NBP2-23326

Formulation	PBS (pH 7.4), 50% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHOSPHO1

Phosphoethanolamine/phosphocholine phosphatase, also known as PHOSPHO1, has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). This protein is involved in the generation of inorganic phosphate for bone mineralization. Recombinant human PHOSPHO1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 3.1.3.75, phosphatase, orphan 1, phosphoethanolamine/phosphocholine phosphatase

Gene Symbol

PHOSPHO1

Additional PHOSPHO1 Products

Product Documents for Recombinant Human PHOSPHO1 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human PHOSPHO1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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