Phototrexate

Tocris Bioscience | Catalog # 7362

Photoswitchable inhibitor of human dihydrofolate reductase
Tocris Bioscience

Key Product Details

Description

Photoswitchable inhibitor of human dihydrofolate reductase
View all Dihydrofolate Reductase Inhibitors »

Product Description

Phototrexate is a photoswitchable potent inhibitor of human dihydrofolate reductase (DHFR). Phototrexate is a photochromic analog of Methotrexate (Cat. No. 1230). Antifolate activity is switched on (cis-Phototrexate) by UVA (375 nm) light; it can be switched off (trans-Phototrexate) by blue or white light and by thermal relaxation in the dark. cis-Phototrexate (IC50 = 6 nM) significantly reduces the viability of HeLa cells compared with trans-Phototrexate (IC50 = 34 μM).

Scientific Data Images for Phototrexate

Photoswitching activity of Phototrexate

Photoswitching activity of Phototrexate

Antifolate activity is switched on (cis-Phototrexate) by UVA (375 nm) light; it can be switched off (trans-Phototrexate) by blue or white light and by thermal relaxation in the dark.

Licensing Information

Sold with permission of IBEC.

Product Specifications for Phototrexate

Molecular Weight

437.42

Formula

C20H19N7O5

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

N-[4-[(1E)-2-(2,4-Diamino-6-quinazolinyl)diazenyl]benzoyl]-L-glutamic acid

CAS Number

2268033-83-4

PubChem ID

142608675

InChI Key

IODLJULYGHWOLC-HIUCBWPRSA-N

SMILES

NC1=NC2=C(C(N)=N1)C=C(/N=N/C3=CC=C(C(N[C@H](C(O)=O)CCC(O)=O)=O)C=C3)C=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 1.75 4

Preparing Stock Solutions for Phototrexate

The following data is based on the product molecular weight 437.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.04 mM 57.15 mL 285.77 mL 571.53 mL
0.2 mM 11.43 mL 57.15 mL 114.31 mL
0.4 mM 5.72 mL 28.58 mL 57.15 mL
2 mM 1.14 mL 5.72 mL 11.43 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for Phototrexate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Phototrexate

For research use only

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