Mouse PIBF1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-68097

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse PIBF in samples. No significant cross-reactivity or interference between Mouse PIBF and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.37%

Inter-Assay Precision (Precision between assays) CV% 5.24%

Recovery for Mouse PIBF1 ELISA Kit (Colorimetric)

Recovery

90-107%

Linearity

Mouse PIBF1 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse PIBF1 ELISA Kit (Colorimetric)

Mouse PIBF1 ELISA Kit (Colorimetric)

ELISA: Mouse PIBF1 ELISA Kit (Colorimetric) [NBP2-68097] -

ELISA: Mouse PIBF1 ELISA Kit (Colorimetric) [NBP2-68097] - Standard Curve Reference

Kit Contents for Mouse PIBF1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PIBF1

PIBF (progesterone-induced blocking factor 1) is synthesized during pregnancy in response to progesterone by progesterone receptor-positive T lymphocytes (mostly gamma-delta T cells). In the presence of PIBF, natural killer (NK) cells inhibit the release of perforin from storage granules and, therefore, fail to lyse target cells. In humans, the amount of cells that express PIBF is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. PIBF has a predicted 89 kDa molecular mass; the full-length PIBF is associated with the nucleus, whereas secretion of shorter forms, such a 34 kDa protein is induced by activation of the cell. Research suggests that PIBF functions as a transcription factor in its full-length form, while smaller forms may act as cytokines.

Long Name

Progesterone Immunomodulatory Binding Factor 1

Alternate Names

C13orf24, chromosome 13 open reading frame 24, KIAA1008, PIBF, progesterone immunomodulatory binding factor 1, progesterone-induced blocking factor 1, progesterone-induced-blocking factor 1, RP11-505F3.1

Gene Symbol

PIBF1

Additional PIBF1 Products

Product Documents for Mouse PIBF1 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse PIBF1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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