Piccolo Antibody (6H9-B6) - BSA Free

Novus Biologicals | Catalog # NBP1-49453

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 6H9-B6

Format

BSA Free
View all Piccolo Primary Antibodies »

Product Specifications

Immunogen

Full length protein, BC001304.1

Reactivity Notes

No cross-reactivity to Bassoon.

Localization

Cell Junction, Synapse

Marker

Synaptic Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

550 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Piccolo Antibody (6H9-B6) - BSA Free

Western Blot: Piccolo Antibody (6H9-B6) [NBP1-49453]

Western Blot: Piccolo Antibody (6H9-B6) [NBP1-49453]

Western Blot: Piccolo Antibody (6H9-B6) [NBP1-49453] - Western Blot analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (NBP1-49453). Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (NBP1-49453) at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate.
Immunocytochemistry/ Immunofluorescence: Piccolo Antibody (6H9-B6) [NBP1-49453]

Immunocytochemistry/ Immunofluorescence: Piccolo Antibody (6H9-B6) [NBP1-49453]

Immunocytochemistry/Immunofluorescence: Piccolo Antibody (6H9-B6) [NBP1-49453] - Analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Piccolo Antibody (D) Composite.
Western Blot: Piccolo Antibody (6H9-B6) [NBP1-49453]

Western Blot: Piccolo Antibody (6H9-B6) [NBP1-49453]

Western Blot: Piccolo Antibody (6H9-B6) [NBP1-49453] - analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate.

Applications for Piccolo Antibody (6H9-B6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of Piccolo Antibody was sufficient for detection of Piccolo in 20 ug of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Piccolo

Piccolo is a presynaptic cytomatrix protein concentrated at the presynaptic side of synaptic junctions. Piccolo is a large protein which constists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. It is usually found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1. Piccolo is highly expressed in brain with low levels found in stomach. It is not detected in other tissues analyzed including adrenal gland, testis and pancreas.

Long Name

Presynaptic Cytomatrix Protein

Alternate Names

ACZ, Aczonin, PCLO

Entrez Gene IDs

27445 (Human)

Gene Symbol

PCLO

UniProt

BC001304.1 (Human)

Additional Piccolo Products

Product Documents for Piccolo Antibody (6H9-B6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Piccolo Antibody (6H9-B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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