PIK3IP1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-69623
![Western Blot: PIK3IP1 Antibody [NBP1-69623]](https://resources.rndsystems.com/images/products/PIK3IP1-Antibody-Western-Blot-NBP1-69623-img0002.jpg "Western Blot: PIK3IP1 Antibody [NBP1-69623]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptides corresponding to PIK3IP1(phosphoinositide-3-kinase interacting protein 1) The peptide sequence was selected from the middle region of human PIK3IP1.
Peptide sequence QALPAFTTEIQEASEGPGADEVQVFAPANALPARSEAAAVQPVIGISQRV. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for PIK3IP1 Antibody - BSA Free
Western Blot: PIK3IP1 Antibody [NBP1-69623]
Western Blot: PIK3IP1 Antibody [NBP1-69623] - Sample Tissue: Human Lung Tumor Antibody Dilution: 1.0 ug/ml![Western Blot: PIK3IP1 Antibody [NBP1-69623]](https://resources.rndsystems.com/images/products/PIK3IP1-Antibody-Western-Blot-NBP1-69623-img0001.jpg "Western Blot: PIK3IP1 Antibody [NBP1-69623]")
Western Blot: PIK3IP1 Antibody [NBP1-69623]
Western Blot: PIK3IP1 Antibody [NBP1-69623] - This Anti-PIK3IP1 antibody was used in Western Blot of HCT15 tissue lysate at a concentration of 1ug/ml.
Western Blot: PIK3IP1 Antibody - BSA Free [NBP1-69623] -
PIK3IP1 plays an important role in the synergistic anti-AML activity upon the combination therapy. (A,B) RNA-seq identified different expression genes (DEGs) of DZNeP or Ven treatment compared to the vehicle control in U937 cells. RNA-seq was performed with total RNA isolated from U937 cells treated with 2 μM of DZNeP, 7.5 μM of Ven, or vehicle for 48 h. (C,D) RNA-seq analysis identified 427 overlapped DEGs; 135 genes are regulated in the same direction, and 292 genes are regulated in the opposite, among which PIK3IP1 was induced by DNZeP while inhibited by Ven. (E) KEGG enrichment analysis of the overlapped DEGs identified the involved pathways co-regulated by DZNeP and Ven. (F) The mRNA expression level of PIK3IP1 induced by DZNeP or/and Ven was measured by qRT-PCR. *, p < 0.05, **, p < 0.01. ***, p < 0.001. ****, p < 0.0001. (G) The protein expression of PIK3IP1 induced by DZNeP or/and Ven was detected by western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36232694), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Western Blot: PIK3IP1 Antibody - BSA Free [NBP1-69623] -
The mechanism of the synergy of co-targeting EZH2 and BCL-2 by DZNeP + Ven is in a PIK3IP1-dependent manner. (A) qRT-PCR was used to measure the efficiency of PIK3IP1 knockdown by shRNA in MV4-11 and U937 AML cells. (B) Western blot was performed to measure the efficiency of PIK3IP1 knockdown by shRNA in MV4-11 and U937 AML cells. (C,D) Effects of the single agents upon proliferation arrest were markedly dampened after PIK3IP1 was knocked down in MV4-11 and U937 AML cells. (E,F) Synergistic effects of the combination treatment upon growth inhibition were significantly impaired when PIK3IP1 was knocked down in MV4-11 and U937 cells. (G,H) Synergistic effects of the combination treatment upon apoptosis were significantly decreased in PIK3IP1 knockdown cells compared to the vector-transfected cells. ns, no significant. *, p < 0.05. ***, p < 0.001. **** p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36232694), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Flow Cytometry: PIK3IP1 Antibody - BSA Free [NBP1-69623] -
PIK3IP1 plays an important role in the synergistic anti-AML activity upon the combination therapy. (A,B) RNA-seq identified different expression genes (DEGs) of DZNeP or Ven treatment compared to the vehicle control in U937 cells. RNA-seq was performed with total RNA isolated from U937 cells treated with 2 μM of DZNeP, 7.5 μM of Ven, or vehicle for 48 h. (C,D) RNA-seq analysis identified 427 overlapped DEGs; 135 genes are regulated in the same direction, and 292 genes are regulated in the opposite, among which PIK3IP1 was induced by DNZeP while inhibited by Ven. (E) KEGG enrichment analysis of the overlapped DEGs identified the involved pathways co-regulated by DZNeP and Ven. (F) The mRNA expression level of PIK3IP1 induced by DZNeP or/and Ven was measured by qRT-PCR. *, p < 0.05, **, p < 0.01. ***, p < 0.001. ****, p < 0.0001. (G) The protein expression of PIK3IP1 induced by DZNeP or/and Ven was detected by western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36232694), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PIK3IP1 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PIK3IP1
Alternate Names
HGFLhHGFL(S), Kringle domain-containing protein HGFL, MGC17330, phosphoinositide-3-kinase interacting protein 1, phosphoinositide-3-kinase-interacting protein 1
Entrez Gene IDs
113791 (Human)
Gene Symbol
PIK3IP1
UniProt
Additional PIK3IP1 Products
Product Documents for PIK3IP1 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for PIK3IP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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