PIP Antibody

Novus Biologicals | Catalog # NB100-2923

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PIP Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GANKAQDNTRK corresponding to internal region according to NP_002643.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PIP Antibody

Western Blot: PIP Antibody [NB100-2923]

Western Blot: PIP Antibody [NB100-2923]

Western Blot: PIP Antibody [NB100-2923] - (0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PIP Antibody [NB100-2923]

Immunohistochemistry-Paraffin: PIP Antibody [NB100-2923]

Immunohistochemistry-Paraffin: PIP Antibody [NB100-2923] - (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PIP Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Paraffin

2 - 3 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 14 kDa band observed in A431 lysates (calculated MW of 16.6 kDa band according to NP_002643.1). IHC-P: Human breast shows staining of luminal epithelial cells of the lactiferous duct.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIP

Gross cystic disease of the breast is benign premenopausal disorder in which cysts are a predominant pathological lesion. These cysts appear to be formed from excessive aporcine cystic secretions. This fluid is composed of several glycoproteins including a unique 15kD monomer protein, PIP. Cytosolic analysis of normal tissue specimens from all major organs has demonstrated PIP in aporcine epithelia, lacrimal, ceruminous and Mollus glands and in numerous serous cells of the submandibular, tracheal, bronchial, sublingual and minor salivary glands. PIP and prostate specific antigen are co-expressed in androgen receptor-positive breast tumors.

Alternate Names

GCDFP15prolactin-inducible protein, GCDFP-15SABP, GPIP4gp17, Gross cystic disease fluid protein 15, prolactin-induced proteinSecretory actin-binding protein

Entrez Gene IDs

5304 (Human)

Gene Symbol

PIP

UniProt

NP_002643.1 (Human)

Additional PIP Products

Product Documents for PIP Antibody

Certificate of Analysis

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Product Specific Notices for PIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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