PITX1 Antibody (5G4) - Azide and BSA Free

Novus Biologicals | Catalog # H00005307-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5G4

Format

Azide and BSA Free
View all PITX1 Primary Antibodies »

Product Specifications

Immunogen

PITX1 (NP_002644, 225 a.a. ~ 313 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPSSMGPGAVPGMPNSGLNNINNLTGSSLNSAMSPGACPYGTPASPYSVYRDTCNSSLASLRLKSKQHSSFGYGGLQGPASGLNACQYN

Specificity

PITX1 - paired-like homeodomain transcription factor 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PITX1 Antibody (5G4) - Azide and BSA Free

Western Blot: PITX1 Antibody (5G4) [H00005307-M01]

Western Blot: PITX1 Antibody (5G4) [H00005307-M01]

Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody (M01), clone 5G4.Lane 1: PITX1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: PITX1 Antibody (5G4) [H00005307-M01]

Western Blot: PITX1 Antibody (5G4) [H00005307-M01]

Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi ( Cat # H00005307-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody (M01), clone 5G4 (Cat # H00005307-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: PITX1 Antibody (5G4) [H00005307-M01]

Western Blot: PITX1 Antibody (5G4) [H00005307-M01]

Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - PITX1 monoclonal antibody (M01), clone 5G4 Analysis of PITX1 expression in Hela S3 NE.
Western Blot: PITX1 Antibody (5G4) [H00005307-M01]

Western Blot: PITX1 Antibody (5G4) [H00005307-M01]

Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 transfected lysate using anti-PITX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PITX1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: PITX1 Antibody (5G4) [H00005307-M01] - Detection limit for recombinant GST tagged PITX1 is approximately 0.03ng/ml as a capture antibody.

Applications for PITX1 Antibody (5G4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PITX1

This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.

Alternate Names

BFTpituitary homeobox 1, CCF, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, paired-like homeodomain 1, pituitary otx-related factor, POTXpituitary homeo box 1, PTX1Paired-like homeodomain transcription factor 1hindlimb expressed homeobox protein backfoot

Entrez Gene IDs

5307 (Human)

Gene Symbol

PITX1

OMIM

602149 (Human)

UniProt

NP_002644 (Human)

Additional PITX1 Products

Product Documents for PITX1 Antibody (5G4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PITX1 Antibody (5G4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PITX1 Antibody (5G4) - Azide and BSA Free

Customer Reviews for PITX1 Antibody (5G4) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review PITX1 Antibody (5G4) - Azide and BSA Free and earn rewards!

Have you used PITX1 Antibody (5G4) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies