PITX2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00005308-D01P

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all PITX2 Primary Antibodies »

Product Specifications

Immunogen

PITX2 (NP_700476.1, 1 a.a. - 271 a.a.) full-length human protein. METNCRKLVSACVQLEKDKSQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPYDDMYPGYSYNNWAAKGLTSASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISSMSMSSSMVPSAVTGVPGSSLNSLNNLNNLSSPSLNSAVPTPACPYAPPTPPYVYRDTCNSSLASLRLKAKQHSSFGYASVQNPASNLSACQYAVDRPV

Specificity

PITX2 - paired-like homeodomain 2,

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for PITX2 Antibody - Azide and BSA Free

Western Blot: PITX2 Antibody [H00005308-D01P]

Western Blot: PITX2 Antibody [H00005308-D01P]

Western Blot: PITX2 Antibody [H00005308-D01P] - Analysis of PITX2 expression in human liver.
Western Blot: PITX2 Antibody [H00005308-D01P]

Western Blot: PITX2 Antibody [H00005308-D01P]

Western Blot: PITX2 Antibody [H00005308-D01P] - Analysis of PITX2 expression in transfected 293T cell line by PITX2 polyclonal antibody.Lane 1: PITX2 transfected lysate(30.30 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Assay: PITX2 Antibody [H00005308-D01P]

Proximity Ligation Assay: PITX2 Antibody [H00005308-D01P]

Proximity Ligation Assay: PITX2 Antibody [H00005308-D01P] - Analysis of protein-protein interactions between PITX2 and CTNNB1. HeLa cells were stained with anti-PITX2 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for PITX2 Antibody - Azide and BSA Free

Application
Recommended Usage

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PITX2

This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Long Name

Pituitary Homeobox 2

Alternate Names

Brx1, IDG2, IGDS, IGDS2, IHG2, IRID2, Otlx2, RGS, RIEG, RIEG1, RP1, Solurshin

Entrez Gene IDs

5308 (Human)

Gene Symbol

PITX2

UniProt

NP_700476.1 (Human)

Additional PITX2 Products

Product Documents for PITX2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PITX2 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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