The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. Catalytic PKA subunit isoforms, Cα, Cβ and, in humans, Cγ, have been identified.
PKA C alpha/beta Antibody (515741) [Janelia Fluor® 646]
Novus Biologicals | Catalog # FAB5908J
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Janelia Fluor 646
Antibody Source
Monoclonal Mouse IgG2A Clone # 515741
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Product Specifications
Immunogen
E. coli-derived recombinant human PKA C alpha
Met1-Phe351
Accession # P17612
Met1-Phe351
Accession # P17612
Specificity
Detects human, mouse, and rat PKA C alpha / beta in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for PKA C alpha/beta Antibody (515741) [Janelia Fluor® 646]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: PKA C alpha/beta
Long Name
cAMP-dependent Protein Kinase Catalytic Subunit alpha/beta
Alternate Names
PRKACA/B
Gene Symbol
PRKACA
Additional PKA C alpha/beta Products
Product Documents for PKA C alpha/beta Antibody (515741) [Janelia Fluor® 646]
Product Specific Notices for PKA C alpha/beta Antibody (515741) [Janelia Fluor® 646]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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