PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)

Novus Biologicals | Catalog # NBP3-32788

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE45-17 expressed in HEK293
View all PKA C (pan) Primary Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr197 of PKA C (pan). (Uniprot: P17612)

Modification

p Thr 197

Localization

Cytoplasm, Cell membrane, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)

PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)

Western Blot: PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) [NBP3-32788]

Western Blot: PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) [NBP3-32788] - Western blot analysis of PKA C (pan) on different lysates with Rabbit anti-PKA C (pan) antibody (NBP3-32788) at 1/1,000 dilution.

Lane 1: NIH/3T3 cell lysate
Lane 2: C6 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 46 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32788) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKA C (pan)

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. Catalytic PKA subunit isoforms, Cα, Cβ and, in humans, Cγ, have been identified.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit

Alternate Names

CAFD1, PKACA, PPNAD4

Gene Symbol

PRKACA

Additional PKA C (pan) Products

Product Documents for PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)

Certificate of Analysis

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Product Specific Notices for PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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