PKA R2 [p Ser99] Antibody (SR1905) - BSA Free

Novus Biologicals | Catalog # NBP3-21511

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1905 expressed in HEK293

Format

BSA Free
View all PKA R2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-PKA R2 [Ser99] (Uniprot #: P13861)

Modification

p Ser 99

Specificity

Detects endogenous levels of total phospho-PKA R2 [Ser99]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKA R2 [p Ser99] Antibody (SR1905) - BSA Free

Western Blot: PKA R2 [p Ser 99] Antibody (SR1905) [NBP3-21511] -

Western Blot: PKA R2 [p Ser 99] Antibody (SR1905) [NBP3-21511] - Western blot analysis using NBP3-21511 in K562 cell lysate.

Applications for PKA R2 [p Ser99] Antibody (SR1905) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKA R2

PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).

Alternate Names

protein kinase, cAMP-dependent, regulatory, type II, alpha, RII-alpha subunit

Gene Symbol

PRKAR2A

Additional PKA R2 Products

Product Documents for PKA R2 [p Ser99] Antibody (SR1905) - BSA Free

Certificate of Analysis

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Product Specific Notices for PKA R2 [p Ser99] Antibody (SR1905) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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