PKC eta Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30454

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PKC eta Primary Antibodies »

Product Specifications

Immunogen

Between 300 and 350

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC eta Antibody - BSA Free

PKC eta Antibody

Immunoprecipitation:PKC eta AntibodyNBP3-30454] -

Immunoprecipitation:PKC eta AntibodyNBP3-30454] -Detection of human PKC-eta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-PKC-eta antibody NBP3-30454 (lot NBP3-30454-1) used for IP at 6 µg/mg lysate. PKC-eta was also immunoprecipitated by rabbit anti-PKC-eta.. For blotting immunoprecipitated PKC-eta, A304-236A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for PKC eta Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PKC eta

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

Long Name

Protein kinase C eta type

Alternate Names

EC 2.7.11.13, nPKC-eta, PKC-L, PKCL, PRKCH

Gene Symbol

PRKCH

Additional PKC eta Products

Product Documents for PKC eta Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PKC eta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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