PKR Antibody (5B7B6)

Novus Biologicals | Catalog # NBP3-15767

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5B7B6 expressed in HEK293
View all PKR Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-551 of human PKR (P19525). GTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKR Antibody (5B7B6)

Western Blot: PKR Antibody (5B7B6) [NBP3-15767]

Western Blot: PKR Antibody (5B7B6) [NBP3-15767]

Western Blot: PKR Antibody (5B7B6) [NBP3-15767] - Western blot analysis of extracts of various cell lines, using PKR antibody (NBP3-15767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for PKR Antibody (5B7B6)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKR

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

Long Name

Protein Kinase R

Alternate Names

EIF2AK2, P68 Kinase, PPP1R83, PRKR, Protein Kinase R

Gene Symbol

EIF2AK2

Additional PKR Products

Product Documents for PKR Antibody (5B7B6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PKR Antibody (5B7B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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