PKR Antibody (6H3A10) - BSA Free

Novus Biologicals | Catalog # NBP2-37242

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6H3A10

Format

BSA Free
View all PKR Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PKR (AA: 329-551) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKR Antibody (6H3A10) - BSA Free

Western Blot: PKR Antibody (6H3A10)BSA Free [NBP2-37242]

Western Blot: PKR Antibody (6H3A10)BSA Free [NBP2-37242]

Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using PKR mAb against (1) HEK293 and (2) PKR (AA: 329-551)-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) - BSA Free [NBP2-37242]

Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) - BSA Free [NBP2-37242]

Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of cervical cancer tissues using EIF2AK2 mouse mAb with DAB staining.
Flow Cytometry: PKR Antibody (6H3A10) - BSA Free [NBP2-37242]

Flow Cytometry: PKR Antibody (6H3A10) - BSA Free [NBP2-37242]

Flow Cytometry: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).
Western Blot: PKR Antibody (6H3A10)BSA Free [NBP2-37242]

Western Blot: PKR Antibody (6H3A10)BSA Free [NBP2-37242]

Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Western blot analysis using EIF2AK2 mAb against human EIF2AK2 (AA: 329-551) recombinant protein. (Expected MW is 51.7 kDa)
Western Blot: PKR Antibody (6H3A10)BSA Free [NBP2-37242]

Western Blot: PKR Antibody (6H3A10)BSA Free [NBP2-37242]

Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using EIF2AK2 mouse mAb against A431 (1), THP-1 (2), MCF-7 (3), PC-12 (4) cell lysate.
Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) - BSA Free [NBP2-37242]

Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) - BSA Free [NBP2-37242]

Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of rectum cancer tissues using EIF2AK2 mouse mAb with DAB staining.
ELISA: PKR Antibody (6H3A10) - BSA Free [NBP2-37242]

ELISA: PKR Antibody (6H3A10) - BSA Free [NBP2-37242]

ELISA: PKR Antibody (6H3A10) [NBP2-37242] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PKR Antibody (6H3A10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKR

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

Long Name

Protein Kinase R

Alternate Names

EIF2AK2, P68 Kinase, PPP1R83, PRKR, Protein Kinase R

Gene Symbol

EIF2AK2

Additional PKR Products

Product Documents for PKR Antibody (6H3A10) - BSA Free

Certificate of Analysis

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Product Specific Notices for PKR Antibody (6H3A10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

Citations for PKR Antibody (6H3A10) - BSA Free

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Protocols

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FAQs

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