Secretory phopholipase A2 (PLA2) enzymes cleave an acyl ester bond in the sn-2 position of glycerophospholipids. These extracellular proteins have a high disulfide bond content, low molecular mass (14 kDa), and require mM levels of Ca2+ for catalysis. They play a crucial role in the generation of arachidonates and eicosanoids, and have a number of biological actions including immunological responses, inflammation, cellular proliferation, vasoconstriction, and bronchioconstriction.
PLA2G4A Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-29928
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Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Between 425 and 475
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PLA2G4A Antibody - BSA Free
Immunoprecipitation:PLA2G4A AntibodyNBP3-29928] -
Immunoprecipitation:PLA2G4A AntibodyNBP3-29928] -Detection of human PLA2G4A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLA2G4A antibody NBP3-29928 (lot NBP3-29928-1) used for IP at 6 µg per reaction. PLA2G4A was also immunoprecipitated by rabbit anti-PLA2G4A.. For blotting immunoprecipitated PLA2G4A, NBP3-29928 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for PLA2G4A Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate pH 7 -8.
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PLA2G4A
Long Name
Phospholipase A2 Group IV A
Alternate Names
cPLA2, cPLA2-alpha
Gene Symbol
PLA2G4A
Additional PLA2G4A Products
Product Documents for PLA2G4A Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for PLA2G4A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
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FAQs for PLA2G4A Antibody - BSA Free
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Q: Hi. Actually I want to purchase anti cPLA2 antibody raised in rabbit which can bind to both phorphorylated and unphosphorylated mouse cPLA2 group IV. I want to a gel shift assay with western blotting to show that my protein of interest is causing the phosphorylation of cPLA2 inside the mouse macrophages RAW cells.Can you please tell me which antibody would be suitable for the purpose. Thanks a lot.
A: Here are our antibodies raised in rabbit that have been validated to detect cPLA2 in mouse samples in a western blot. We have not specifically confirmed the ability of these antibodies to detect both the phosphorylated and unphosphorylated forms of the protein, and I cannot guarantee that they will clearly differentiate between the two.
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Associated Pathways
