PLD3/Phospholipase D3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35842

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PLD3/Phospholipase D3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 279-490 of human PLD3/Phospholipase D3 (NP_001026866).

Sequence:
MEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYVAVMNYLPTLEFSHPHRFWPAIDDGLRRATYERGVKVRLLISCWGHSEPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADEAQARIPYARVNHNKYMVTERATYIGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYSHDLDTSADSVGNACRLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLD3/Phospholipase D3 Antibody - BSA Free

PLD3/Phospholipase D3 Antibody

Immunocytochemistry/ Immunofluorescence: PLD3/Phospholipase D3 Antibody [NBP3-35842] -

Immunocytochemistry/ Immunofluorescence: PLD3/Phospholipase D3 Antibody [NBP3-35842] - Immunofluorescence analysis of L929 cells using PLD3/Phospholipase D3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
PLD3/Phospholipase D3 Antibody

Western Blot: PLD3/Phospholipase D3 Antibody [NBP3-35842] -

Western Blot: PLD3/Phospholipase D3 Antibody [NBP3-35842] - Western blot analysis of various lysates using PLD3/Phospholipase D3 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Applications for PLD3/Phospholipase D3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLD3/Phospholipase D3

Alternate Names

Choline phosphatase 3, EC 3.1.4, EC 3.1.4.4, HindIII K4L homolog, HUK4, HU-K4, Phosphatidylcholine-hydrolyzing phospholipase D3, phospholipase D family, member 3, phospholipase D3, PLD 3

Gene Symbol

PLD3

Additional PLD3/Phospholipase D3 Products

Product Documents for PLD3/Phospholipase D3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PLD3/Phospholipase D3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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