PLS1 Antibody (3G10) - Azide and BSA Free

Novus Biologicals | Catalog # H00005357-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3G10

Format

Azide and BSA Free
View all PLS1 Primary Antibodies »

Product Specifications

Immunogen

PLS1 (NP_002661.1, 1 a.a. ~ 102 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MENSTTTISREELEELQEAFNKIDIDNSGYVSDYELQDLFKEASLPLPGYKVREIVEKILSVADSNKDGKISFEEFVSLMQELKSKDISKTFRKIINKREGI

Specificity

PLS1 - plastin 1 (I isoform) (3G10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PLS1 Antibody (3G10) - Azide and BSA Free

Western Blot: PLS1 Antibody (3G10) [H00005357-M04]

Western Blot: PLS1 Antibody (3G10) [H00005357-M04]

Western Blot: PLS1 Antibody (3G10) [H00005357-M04] - Analysis of PLS1 expression in Jurkat (Cat # L017V1).
ELISA: PLS1 Antibody (3G10) [H00005357-M04]

ELISA: PLS1 Antibody (3G10) [H00005357-M04]

ELISA: PLS1 Antibody (3G10) [H00005357-M04] - Detection limit for recombinant GST tagged PLS1 is approximately 3ng/ml as a capture antibody.

Applications for PLS1 Antibody (3G10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Knock Out Validation was reported in scientific literature (PMID: 27811163).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLS1

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. Fimbrin is a 68-kD actin-bundling protein. [provided by RefSeq]

Alternate Names

fimbrin, I plastin, intestine specific plastin, Intestine-specific plastin, I-plastin, plastin 1, plastin 1 (I isoform), Plastin-1

Entrez Gene IDs

5357 (Human)

Gene Symbol

PLS1

UniProt

NP_002661 (Human)

Additional PLS1 Products

Product Documents for PLS1 Antibody (3G10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PLS1 Antibody (3G10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PLS1 Antibody (3G10) - Azide and BSA Free

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Protocols

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FAQs

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