PMEL17/SILV Antibody (PMEL/2038) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-08517
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, ELISA, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PMEL/2038
Format
Azide and BSA Free
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Product Specifications
Immunogen
A recombinant fragment (around aa 376-502) of human SILV protein (exact sequence is proprietary) (Uniprot: P40967)
Localization
Cytoplasmic
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07599).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for PMEL17/SILV Antibody (PMEL/2038) - Azide and BSA Free
Western Blot: PMEL17/SILV Antibody (PMEL/2038)Azide and BSA Free [NBP3-08517]
Western Blot: PMEL17/SILV Antibody (PMEL/2038) [NBP3-08517] - Western Blot Analysis of COLO-38 cell lysate using PMEL17/SILV Mouse Monoclonal Antibody (PMEL/2038).Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2038) - Azide and BSA Free [NBP3-08517]
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2038) [NBP3-08517] - Formalin-fixed, paraffin-embedded human Skin stained with PMEL17/SILV Mouse Monoclonal Antibody (PMEL/2038).Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2038) - Azide and BSA Free [NBP3-08517]
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2038) [NBP3-08517] - Formalin-fixed, paraffin-embedded human Melanoma stained with PMEL17/SILV Mouse Monoclonal Antibody (PMEL/2038).Protein Array: PMEL17/SILV Antibody (PMEL/2038) - Azide and BSA Free [NBP3-08517]
Protein Array: PMEL17/SILV Antibody (PMEL/2038) [NBP3-08517] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PMEL17/SILV Mouse Monoclonal Antibody (PMEL/2038).Applications for PMEL17/SILV Antibody (PMEL/2038) - Azide and BSA Free
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
2-4 ug/ml
Application Notes
ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: PMEL
Long Name
Premelanosome Protein
Alternate Names
Gp100, ME20, ME20-M, ME20M, PMEL17, SILV
Gene Symbol
PMEL
Additional PMEL Products
Product Documents for PMEL17/SILV Antibody (PMEL/2038) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PMEL17/SILV Antibody (PMEL/2038) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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