PMEL17/SILV Antibody

Novus Biologicals | Catalog # NB100-41098

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence CPIGENSPLLSGQQ corresponding to C-Terminus according to NP_008859.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24572365).

Marker

Melanosome Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PMEL17/SILV Antibody

Western Blot: PMEL17/SILV Antibody [NB100-41098]

Western Blot: PMEL17/SILV Antibody [NB100-41098]

Western Blot: PMEL17/SILV Antibody [NB100-41098] - Staining of Mouse Skin lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PMEL17/SILV Antibody [NB100-41098]

Immunocytochemistry/ Immunofluorescence: PMEL17/SILV Antibody [NB100-41098]

Immunocytochemistry/Immunofluorescence: PMEL17/SILV Antibody [NB100-41098] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for PMEL17/SILV Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 26 kDa band observed in human skin lysates, corresponding to the M-beta fragment of the precursor protein, reported in scientific literature (PMID: 23389629). Calculated MW of 70.3 kDa band according to NP_008859.1

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PMEL

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Entrez Gene IDs

6490 (Human); 20431 (Mouse); 362818 (Rat)

Gene Symbol

PMEL

UniProt

P40967 (Human)

Additional PMEL Products

Product Documents for PMEL17/SILV Antibody

Certificate of Analysis

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Product Specific Notices for PMEL17/SILV Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PMEL17/SILV Antibody

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Protocols

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