Chemical Name: Phenylmethylsulfonyl fluoride
Biological ActivityPMSF is a non-selective protease inhibitor; irreversibly sulfonates key active site serine residues in serine proteases, acetylcholinesterases, and thiol proteases. Blocks metabolism of anandamide (Cat. No. 1339) in a rodent model of cannabimimetic activity. Unstable in water (t1/2 ~ 60 mins at pH 7.5). Brain penetrant.
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For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotrypsin and trypsin.
Turini et al.
The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity.
Compton & Martin
Role of fatty acid amide hydrolase in the transport of the endogenous cannabinoid anandamide.
Day et al.
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Used to isolate protein from kidney tissue from wild type or IL-33 deficient mice that underwent cisplatin-induced kidney injury; several proteins were detected by immunoblot analysis; even after several freeze thaws, data has been consistent*additionally added a protease inhibitor cocktail and phosphatase inhibitor cocktail + RIPA for protein isolation