Podocin/NPHS2 Antibody (2O10G3)

Novus Biologicals | Catalog # NBP3-16111

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2O10G3 expressed in HEK293
View all Podocin/NPHS2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 284-383 of human Podocin/NPHS2 (Q9NP85). AQRQAKVRMIAAEAEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSKPVEPLNPKKKDSPML

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Podocin/NPHS2 Antibody (2O10G3) (NBP3-16111) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Podocin/NPHS2 Antibody (2O10G3)

Western Blot: Podocin/NPHS2 Antibody (2O10G3) [NBP3-16111]

Western Blot: Podocin/NPHS2 Antibody (2O10G3) [NBP3-16111]

Western Blot: Podocin/NPHS2 Antibody (2O10G3) [NBP3-16111] - Western blot analysis of extracts of various cell lines, using Podocin/NPHS2 antibody (NBP3-16111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Applications for Podocin/NPHS2 Antibody (2O10G3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Podocin/NPHS2

NPHS2 encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Alternate Names

Nephrosis 2, NPHS2, PDCN, SRN1

Gene Symbol

NPHS2

Additional Podocin/NPHS2 Products

Product Documents for Podocin/NPHS2 Antibody (2O10G3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Podocin/NPHS2 Antibody (2O10G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Immunoglobulin Superfamily CAMs

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