POLD3 Antibody (3E2) - Azide and BSA Free

Novus Biologicals | Catalog # H00010714-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E2

Format

Azide and BSA Free
View all POLD3 Primary Antibodies »

Product Specifications

Immunogen

POLD3 (NP_006582, 357 a.a. ~ 466 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPLEPVPKTEPEPPSVKSSSGENKRKRKRVLKSKTYLDGEGCIVTEKVYESESCTDSEEELNMKTSSVHRPPAMTVKKEPREERKGPKKGTAALGKANRQVSITGFFQRK

Specificity

POLD3 - polymerase (DNA-directed), delta 3, accessory subunit

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for POLD3 Antibody (3E2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: POLD3 Antibody (3E2) [H00010714-M01]

Immunocytochemistry/ Immunofluorescence: POLD3 Antibody (3E2) [H00010714-M01]

Immunocytochemistry/Immunofluorescence: POLD3 Antibody (3E2) [H00010714-M01] - Analysis of monoclonal antibody to POLD3 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: POLD3 Antibody (3E2) [H00010714-M01]

ELISA: POLD3 Antibody (3E2) [H00010714-M01]

ELISA: POLD3 Antibody (3E2) [H00010714-M01] - Detection limit for recombinant GST tagged POLD3 is approximately 0.03ng/ml as a capture antibody.

Applications for POLD3 Antibody (3E2) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POLD3

The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1 (MIM 174761), POLD2 (MIM 600815), POLD3, and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM]

Alternate Names

DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, DNA polymerase delta, subunit 3, KIAA0039MGC119643, MGC119642, P66, P68, polymerase (DNA directed), delta 3, polymerase (DNA-directed), delta 3, accessory subunit

Entrez Gene IDs

10714 (Human)

Gene Symbol

POLD3

UniProt

NP_006582 (Human)

Additional POLD3 Products

Product Documents for POLD3 Antibody (3E2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for POLD3 Antibody (3E2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for POLD3 Antibody (3E2) - Azide and BSA Free

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Protocols

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FAQs

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