POLD3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93032

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1). ASKAAAKTQETNKETKTEAKEVTNASAAGNKAPGKGNMMSNFFGKAAMNKFKVNLDSEQAVKEEKIVEQPTVSVTEPKLATPAGLKKSSKKAEPVKVLQKEKKRGKRVALSDDETKETENMRKKRRRIKLPESDSSEDEVFPDSPGAYEAESPSPPPPPSPPLEPVPKTEPEPPSVKSSSGENKRKRKRVLKSKTYLDGEGCIVTEKVYESESCTDSEEELNMKTSSVHRPPAMTVKKEPREERKGPKKGTAALGKANRQVSITGFFQRK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLD3 Antibody - BSA Free

Western Blot: POLD3 AntibodyBSA Free [NBP2-93032]

Western Blot: POLD3 AntibodyBSA Free [NBP2-93032]

Western Blot: POLD3 Antibody [NBP2-93032] - Analysis of extracts of various cell lines, using POLD3. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: POLD3 Antibody - BSA Free [NBP2-93032]

Immunocytochemistry/ Immunofluorescence: POLD3 Antibody - BSA Free [NBP2-93032]

Immunocytochemistry/Immunofluorescence: POLD3 Antibody [NBP2-93032] - Analysis of U2OS cells using POLD3.
Immunocytochemistry/ Immunofluorescence: POLD3 Antibody - BSA Free [NBP2-93032]

Immunocytochemistry/ Immunofluorescence: POLD3 Antibody - BSA Free [NBP2-93032]

Immunocytochemistry/Immunofluorescence: POLD3 Antibody [NBP2-93032] - Analysis of GFP-RNF168 transgenic U2OS cells using POLD3 can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
POLD3 Antibody - BSA Free

Immunohistochemistry: POLD3 Antibody - BSA Free [NBP2-93032] -

Immunohistochemistry: POLD3 Antibody - BSA Free [NBP2-93032] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using POLD3 Rabbit pAb at dilution of 1:300 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Applications for POLD3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLD3

PolD3/p66 is a subunit of the heterotetrameric DNA polymerase delta that plays a role in DNA replication and repair. The PolD3/p66 subunit has been shown to mediate the interaction between DNA polymerase delta and PCNA (proliferating cell nuclear antigen) to facilitate processivity.

Alternate Names

DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, DNA polymerase delta, subunit 3, KIAA0039MGC119643, MGC119642, P66, P68, polymerase (DNA directed), delta 3, polymerase (DNA-directed), delta 3, accessory subunit

Gene Symbol

POLD3

Additional POLD3 Products

Product Documents for POLD3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for POLD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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