Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POLR2D Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human POLR2D fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for POLR2D Antibody - BSA Free

Western Blot: POLR2D Antibody [NBP2-97517]

Western Blot: POLR2D Antibody [NBP2-97517]

Western Blot: POLR2D Antibody [NBP2-97517] - Anti-POLR2D rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: MCF-7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 18 kDa
Immunoprecipitation: POLR2D Antibody [NBP2-97517]

Immunoprecipitation: POLR2D Antibody [NBP2-97517]

Immunoprecipitation: POLR2D Antibody [NBP2-97517] - POLR2D was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-POLR2D rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-POLR2D rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 17 kDa

Applications for POLR2D Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR2D

The POLR2D gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have

Alternate Names

DNA-directed RNA polymerase II 16 kDa polypeptide, DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit RPB4, HSRBP4, HSRPB4, polymerase (RNA) II (DNA directed) polypeptide D, RBP4, RNA polymerase II 16 kDa subunit, RNA polymerase II subunit B4, RNA polymerase II subunit hsRBP4, RPB16

Gene Symbol

POLR2D

Additional POLR2D Products

Product Documents for POLR2D Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for POLR2D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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