Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage

Name: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage
Brand: Novus Biologicals
SKU: NBP3-16232
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16232

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5Y8J0 expressed in HEK293

View all Poly-Ubiquitin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Poly-Ubiquitin (P0CG47). MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage

Western Blot: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] -

Western Blot: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Western blot analysis of lysates from HeLa cells using Poly-Ubiquitin Rabbit mAb at 1:1000 dilution incubated overnight at 4C. HeLa cells were treated by MG132 (50 uM) at 37C for 90 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Immunocytochemistry/ Immunofluorescence: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] -

Immunocytochemistry/ Immunofluorescence: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Confocal imaging of MCF7 cells using Poly-Ubiquitin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] -

Confocal imaging of HeLa cells (treated with MG-132) and HeLa cells (untreated) cells using K48-linkage Specific Ubiquitin Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Poly-Ubiquitin

Poly-Ubiquitin chains are composed of Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule. Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively. Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes.

Alternate Names

PolyUbiquitin

Gene Symbol

RPS27A

Additional Poly-Ubiquitin Products

Product Documents for Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

NFkB Pathway

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