POMT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86152

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POMT2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GDGFFSSAFQARLSGNNLHNASIPEHLAYGSVITVKNLRMAIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKHNTNSDPLDPSFPVEFVRHGDIIRLEHKETSRNLHSHYHEAPMTRKHYQVTGYGINGT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POMT2 Antibody - BSA Free

Immunohistochemistry-Paraffin: POMT2 Antibody [NBP1-86152]

Immunohistochemistry-Paraffin: POMT2 Antibody [NBP1-86152]

Immunohistochemistry-Paraffin: POMT2 Antibody [NBP1-86152] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
POMT2 Antibody - BSA Free Western Blot: POMT2 Antibody - BSA Free [NBP1-86152]

Western Blot: POMT2 Antibody - BSA Free [NBP1-86152]

Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
POMT2 Antibody - BSA Free Western Blot: POMT2 Antibody - BSA Free [NBP1-86152]

Western Blot: POMT2 Antibody - BSA Free [NBP1-86152]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Applications for POMT2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POMT2

POMT2, also known as Protein-O-Mannosyltransferase 2, is an O-mannosyltransferase that must interact with POMT1 for proper enzymatic function. POMT2 is localized to the Endoplasmic Reticulum membrane, and is ubiquitously expressed at low levels, however highly expressed in the testes. Current research surrounding POMT2 has shown a connection with autosomal recessive muscular dystrophy, as well as possible interactions with Walker-Warburg syndrome, cleft palate, and malaria. POMT2 interacts with POMT1, ALG3, ALG6, ARPC3, and ATP13A1.

Alternate Names

DKFZp686G10254, Dolichyl-phosphate-mannose--protein mannosyltransferase 2, EC 2.4.1.109, FLJ22309, MDDGA2, MDDGB2, MDDGC2, protein O-mannosyl-transferase 2, protein-O-mannosyltransferase 2

Gene Symbol

POMT2

Additional POMT2 Products

Product Documents for POMT2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for POMT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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