PP2C beta/PPM1B Antibody (OTI3F2)

Novus Biologicals | Catalog # NBP2-00720

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F2
View all PP2C beta/PPM1B Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PPM1B (NP_002697) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse PP2C beta/PPM1B Antibody (OTI3F2) (NBP2-00720) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PP2C beta/PPM1B Antibody (OTI3F2)

Western Blot: PPM1B Antibody (3F2) [NBP2-00720] Analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1B monoclonal antibody.
Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3F2) [NBP2-00720]

Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3F2) [NBP2-00720]

Flow Cytometry: PPM1B Antibody (3F2) [NBP2-00720] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPM1B antibody, and then analyzed by flow cytometry.
Western Blot: PP2C beta/PPM1B Antibody (OTI3F2) [NBP2-00720]

Western Blot: PP2C beta/PPM1B Antibody (OTI3F2) [NBP2-00720]

Western Blot: PPM1B Antibody (3F2) [NBP2-00720] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B.
Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3F2) [NBP2-00720]

Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3F2) [NBP2-00720]

Flow Cytometry: PPM1B Antibody (3F2) [NBP2-00720] - Analysis of Hela cells, using anti-PPM1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3F2) [NBP2-00720]

Flow Cytometry: PP2C beta/PPM1B Antibody (OTI3F2) [NBP2-00720]

Flow Cytometry: PPM1B Antibody (3F2) [NBP2-00720] - Analysis of Jurkat cells, using anti-PPM1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for PP2C beta/PPM1B Antibody (OTI3F2)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PP2C beta/PPM1B

PPM1B is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.

Long Name

Protein Phosphatase 2C beta

Alternate Names

Pp2c2, PP2CB, PPM1B

Entrez Gene IDs

5945 (Human)

Gene Symbol

PPM1B

Additional PP2C beta/PPM1B Products

Product Documents for PP2C beta/PPM1B Antibody (OTI3F2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PP2C beta/PPM1B Antibody (OTI3F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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