PP2C epsilon/PPM1L Overexpression Lysate

Novus Biologicals | Catalog # NBL1-14666

Novus Biologicals
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Key Product Details

Species

Human

Applications

Western Blot

Product Summary for PP2C epsilon/PPM1L Overexpression Lysate

PP2C epsilon/PPM1L Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC218909

Accession#: NM_139245

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

40.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for PP2C epsilon/PPM1L Overexpression Lysate

Western Blot: PP2C epsilon/PPM1L Overexpression Lysate [NBL1-14666]

Western Blot: PP2C epsilon/PPM1L Overexpression Lysate [NBL1-14666]

Western Blot: PPM1L Overexpression Lysate (Adult Normal) [NBL1-14666] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPM1L.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: PP2C epsilon/PPM1L

PPM1L, or PP2CE, belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. PP2Cs regulate stress-activated protein kinase (SAPK; see MIM 601158) signaling cascades that respond to extracellular stimuli (Jin et al., 2004 [PubMed 15560375]).[supplied by OMIM]

Long Name

Protein Phosphatase 2C alpha

Alternate Names

PP2CE, PPM1L

Gene Symbol

PPM1L

Additional PP2C epsilon/PPM1L Products

Product Documents for PP2C epsilon/PPM1L Overexpression Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PP2C epsilon/PPM1L Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail