PP5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87240

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PP5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGERTECAEPPRDEPPADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRSLAYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQECNKIVKQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit PP5 Antibody - BSA Free (NBP1-87240) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PP5 Antibody - BSA Free

Western Blot: PP5 Antibody [NBP1-87240]

Western Blot: PP5 Antibody [NBP1-87240]

Western Blot: PP5 Antibody [NBP1-87240] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: PP5 Antibody [NBP1-87240]

Immunocytochemistry/ Immunofluorescence: PP5 Antibody [NBP1-87240]

Immunocytochemistry/Immunofluorescence: PP5 Antibody [NBP1-87240] - Staining of human cell line U-251 MG shows localization to cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Western Blot: PP5 Antibody [NBP1-87240]

Western Blot: PP5 Antibody [NBP1-87240]

Western Blot: PP5 Antibody [NBP1-87240] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human colon shows weak cytoplasmicand moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human prostate shows weak cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of neurons.

Applications for PP5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP5

It may play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, it dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.

Long Name

Protein Phosphatase 5, Catalytic Subunit

Alternate Names

EC 3.1.3.16, PPP5, PPP5C, PPT

Gene Symbol

PPP5C

Additional PP5 Products

Product Documents for PP5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PP5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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