PPP1R9B/Spinophilin Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94055

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all PPP1R9B Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human PPP1R9B/Spinophilin (NP_115984.3). VFQPPPPPPPAPSGDAPAEKERCPAGQQPPQHRVAPARPPPKPREVRKIKPVEVEESGESEAESAPGEVIQAEVTVHAALENGSTVATAASPAPEEPKAQA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPP1R9B/Spinophilin Antibody - Azide and BSA Free

Western Blot: PPP1R9B/Spinophilin AntibodyAzide and BSA Free [NBP2-94055]

Western Blot: PPP1R9B/Spinophilin AntibodyAzide and BSA Free [NBP2-94055]

Western Blot: PPP1R9B/Spinophilin Antibody [NBP2-94055] - Analysis of extracts of various cell lines, using PPP1R9B/Spinophilin at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: PPP1R9B/Spinophilin Antibody - Azide and BSA Free [NBP2-94055]

Immunocytochemistry/ Immunofluorescence: PPP1R9B/Spinophilin Antibody - Azide and BSA Free [NBP2-94055]

Immunocytochemistry/Immunofluorescence: PPP1R9B/Spinophilin Antibody [NBP2-94055] - Analysis of L929 cells using PPP1R9B/Spinophilin at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for PPP1R9B/Spinophilin Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPP1R9B

Spinophilin, also known as Neurabin-2 and PPP1R9B, has 815 amino acids and is approximately 89kDa. Spinophilin is a scaffold protein that is involved in many signaling pathways, such as the cAMP pathway, the G-protein coupled receptor signaling pathway, the IL-6 pathway, and the Beta-Adrenergic signaling pathway. Spinophilin may be implicated in the linking of the plasma membrane to the actin cytoskeleton at synaptic junctions. Current research on Spinophilin is being performed in relation to several diseases and disorders including anorexia nervosa, Alzheimer's disease, schizophrenia, encephalitis, breast cancer, mood disorder, and glioblastoma. Spinophilin has also been shown to have interactions with CCKAR, CCKBR, ADRA1B, DRD2, and PPP1CA.

Long Name

Protein Phosphatase 1 Regulatory Subunit 9B

Alternate Names

Neurabin-2, PPP1R6, SPINO, Spinophilin

Gene Symbol

PPP1R9B

Additional PPP1R9B Products

Product Documents for PPP1R9B/Spinophilin Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PPP1R9B/Spinophilin Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Epigenetics
Phosphatases and Regulators

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Protocols

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FAQs

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