PPP2R4 Antibody

Novus Biologicals | Catalog # NBP1-51951

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PPP2R4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GSLLPIHPVTSG corresponding to C-Terminus according to NP_066954.2, NP_821070.1.

Epitope

C-GSLLPIHPVTSG

Specificity

This antibody is expected to recognize all reported isoforms (NP_821068.1; NP_066954.2; NP_821070.1). Reported variants NP_066954.2 and NP_821067.1 represent identical protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat PPP2R4 Antibody (NBP1-51951) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-PPP2R4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PPP2R4 Antibody

Western Blot: PPP2R4 Antibody [NBP1-51951]

Western Blot: PPP2R4 Antibody [NBP1-51951]

Western Blot: PPP2R4 Antibody [NBP1-51951] - Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-51951]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-51951]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-51951] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PPP2R4 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in human, mouse and rat liver lysates and in HepG2 lysates (calculated MW of 36.8 kDa band according to NP_066954.2).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPP2R4

Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.

Long Name

Serine/threonine Protein Phosphatase 2A Regulatory Subunit B

Alternate Names

PTPA

Entrez Gene IDs

5524 (Human); 110854 (Mouse); 362102 (Rat)

Gene Symbol

PTPA

UniProt

NP_821068.1 (Human)

Additional PPP2R4 Products

Product Documents for PPP2R4 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for PPP2R4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

Citations for PPP2R4 Antibody

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Protocols

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