PPP2R4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87232

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPP2R4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit PPP2R4 Antibody - BSA Free (NBP1-87232) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PPP2R4 Antibody - BSA Free

Western Blot: PPP2R4 Antibody [NBP1-87232]

Western Blot: PPP2R4 Antibody [NBP1-87232]

Western Blot: PPP2R4 Antibody [NBP1-87232] - Analysis in human cell line SiHa.
Immunocytochemistry/ Immunofluorescence: PPP2R4 Antibody [NBP1-87232]

Immunocytochemistry/ Immunofluorescence: PPP2R4 Antibody [NBP1-87232]

Immunocytochemistry/Immunofluorescence: PPP2R4 Antibody [NBP1-87232] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Western Blot: PPP2R4 Antibody [NBP1-87232]

Western Blot: PPP2R4 Antibody [NBP1-87232]

Western Blot: PPP2R4 Antibody [NBP1-87232] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Western Blot: PPP2R4 Antibody [NBP1-87232]

Western Blot: PPP2R4 Antibody [NBP1-87232]

Western Blot: PPP2R4 Antibody [NBP1-87232] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232] - Staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-87232] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts and leydig cells.

Applications for PPP2R4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPP2R4

Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.

Long Name

Serine/threonine Protein Phosphatase 2A Regulatory Subunit B

Alternate Names

PTPA

Gene Symbol

PTPA

Additional PPP2R4 Products

Product Documents for PPP2R4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PPP2R4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

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Protocols

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FAQs

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