PR48 Antibody (2E12) - Azide and BSA Free

Novus Biologicals | Catalog # H00028227-M08

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2E12

Format

Azide and BSA Free
View all PR48 Primary Antibodies »

Product Specifications

Immunogen

PPP2R3B (AAH11180, 1 a.a. ~ 225 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQCRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITLQDLKRCKLANVFFDTFFNIEKYLDHEQKEQISLLRDGDSGGPELSDWEKYAAEEYDILVAEETAGEPWEDGFEAELSPVEQKLSALRSPLAQRPFFEAPSPLGAVDLYEYACGDEDLEPL

Specificity

PPP2R3B - protein phosphatase 2 (formerly 2A), regulatory subunit B', beta (2E12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for PR48 Antibody (2E12) - Azide and BSA Free

Western Blot: PR48 Antibody (2E12) [H00028227-M08]

Western Blot: PR48 Antibody (2E12) [H00028227-M08]

Western Blot: PR48 Antibody (2E12) [H00028227-M08] - Analysis of PPP2R3B transfected lysate using anti-PPP2R3B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPP2R3B rabbit polyclonal antibody.
Immunocytochemistry/ Immunofluorescence: PR48 Antibody (2E12) [H00028227-M08]

Immunocytochemistry/ Immunofluorescence: PR48 Antibody (2E12) [H00028227-M08]

Immunocytochemistry/Immunofluorescence: PR48 Antibody (2E12) [H00028227-M08] - Analysis of monoclonal antibody to PPP2R3B on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: PR48 Antibody (2E12) [H00028227-M08] - Detection limit for recombinant GST tagged PPP2R3B is 0.3 ng/ml as a capture antibody.

Applications for PR48 Antibody (2E12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PR48

Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the beta subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

FLJ60425, NYREN8, NY-REN-8 antigen, PP2A subunit B isoform PR48, PP2A, subunit B, PR48 isoform, PPP2R3L, PPP2R3LY, PR48, protein phosphatase 2 (formerly 2A), regulatory subunit B'', beta, protein phosphatase 2, regulatory subunit B'', beta, Protein phosphatase 2A 48 kDa regulatory subunit, serine/threonine protein phosphatase 2A, 48kDa regulatory subunit B, serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta

Entrez Gene IDs

28227 (Human)

Gene Symbol

PPP2R3B

UniProt

AAH11180 (Human)

Additional PR48 Products

Product Documents for PR48 Antibody (2E12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PR48 Antibody (2E12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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