PRELID2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81937

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QLEEESWLNPRERNMAIRSHCLTWTQYASMKEESVFRESMENPNWTEFIQRGRISITGVGFLNCVLETFASTFLRQGAQKGIRIMEMLLKEQCG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRELID2 Antibody - BSA Free

Western Blot: PRELID2 Antibody [NBP1-81937]

Western Blot: PRELID2 Antibody [NBP1-81937]

Western Blot: PRELID2 Antibody [NBP1-81937] - Analysis in control (vector only transfected HEK293T lysate) and PRELID2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PRELID2 Antibody [NBP1-81937]

Immunocytochemistry/ Immunofluorescence: PRELID2 Antibody [NBP1-81937]

Immunocytochemistry/Immunofluorescence: PRELID2 Antibody [NBP1-81937] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
PRELID2 Antibody

Immunohistochemistry-Paraffin: PRELID2 Antibody [NBP1-81937] -

Immunohistochemistry-Paraffin: PRELID2 Antibody [NBP1-81937] -Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
PRELID2 Antibody - BSA Free

Immunohistochemistry: PRELID2 Antibody - BSA Free [NBP1-81937] -

Elevated PRELID2 is positively correlated with the FBXL6/p-ERK/p-mTOR pathway and poor prognosis of HCC. a Representative images of IHC staining for FBXL6, PRELID2, p-ERK, and p-mTOR in human HCC tumors. Scale bar = 50 um. b The association between PRELID2 and FBXL6, p-ERK, or p-mTOR in 129 paired HCC tumors and adjacent normal tissues was analyzed by χ2 test. c The prognostic significance of PRELID2 in HCC patients was evaluated by Kaplan–Meier analysis. High expression of PRELID2 predicted a shorter overall survival (OS) time. d The association between FBXL6 and p-ERK/p-mTOR protein levels in 129 HCC tissues was evaluated by the χ2 test. e The prognostic significance of the coexpression of FBXL6 and p-ERK in HCC patients was evaluated by Kaplan–Meier analysis. f Kaplan–Meier survival curves showing the overall survival of FBXL6high/p-ERKhigh HCC patients with high or low PRELID2 expression. PRELID2 the proteins of relevant evolutionary and lymphoid interest (PRELI) domain 2, FBXL6 F-box and leucine-rich repeat 6, ERK extracellular signal-regulated kinase, mTOR mammalian target of rapamycin, IHC immunohistochemistry, HCC hepatocellular carcinoma Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38124228), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PRELID2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRELID2

Alternate Names

FLJ38376, MGC21644, PRELI domain containing 2, PRELI domain-containing protein 2

Gene Symbol

PRELID2

Additional PRELID2 Products

Product Documents for PRELID2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PRELID2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PRELID2 Antibody - BSA Free

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Protocols

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FAQs

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