PRKCDBP Antibody

Novus Biologicals | Catalog # NBP1-45210

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PRKCDBP Primary Antibodies »

Product Specifications

Immunogen

PRKCDBP Antibody is made to a peptide with sequence C-RRTGLQKVQSLR corresponding to internal region according to NP_659477.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PRKCDBP Antibody

Western Blot: PRKCDBP Antibody [NBP1-45210]

Western Blot: PRKCDBP Antibody [NBP1-45210]

Western Blot: PRKCDBP Antibody [NBP1-45210] - Staining of Mouse Ovary (A) and Rat Uterus (B) lysate (35 ug protein in RIPA buffer). PRKCDBP antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PRKCDBP Antibody [NBP1-45210]

Immunohistochemistry-Paraffin: PRKCDBP Antibody [NBP1-45210]

Immunohistochemistry-Paraffin: PRKCDBP Antibody [NBP1-45210] - Staining of paraffin embedded Human Uterus. PRKCDBP antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: PRKCDBP Antibody [NBP1-45210]

Western Blot: PRKCDBP Antibody [NBP1-45210]

Western Blot: PRKCDBP Antibody [NBP1-45210] - Staining of Human Adipose cell llysate (35 ug protein in RIPA buffer). PRKCDBP antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PRKCDBP Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 1 ug/mL
Application Notes
Approx 38 kDa band observed in Human Adipose, in Mouse Ovary and Rat Uterus and in NIH3T3 lysates (calculated MW of 27.7 kDa according to Human NP_659477.2, of 27.8 kDa according to Mouse NP_082720.1 and of 27.9 kDa according to Rat NP_604444.1).The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRKCDBP

The protein encoded by this gene was identified as a binding protein of the protein kinase C, delta (PRKCD). The expression of this gene in cultured cell lines is strongly induced by serum starvation. The expression of this protein was found to be down-regulated in various cancer cell lines, suggesting the possible tumor suppressor function of this protein.

Alternate Names

cavin-3, HSRBC, MGC20400, protein kinase C, delta binding protein, sdr-related gene product that binds to c-kinase, Serum deprivation response factor-related gene product that binds to C-kinase, SRBCprotein kinase C delta-binding protein

Entrez Gene IDs

112464 (Human); 109042 (Mouse); 85332 (Rat)

Gene Symbol

CAVIN3

UniProt

Q969G5 (Human)

Additional PRKCDBP Products

Product Documents for PRKCDBP Antibody

Certificate of Analysis

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Product Specific Notices for PRKCDBP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PRKCDBP Antibody

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Protocols

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