ProDots Recombinant Human FGF-3 Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD1206-10
PRD1206-50
PRD1206-01
ProDots Recombinant Human FGF-3 Protein Bioactivity
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ProDots Recombinant Human FGF-3 Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium


Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 0.02-0.1 μg/mL in the presence of 1 μg/mL  of heparin.
Source
E. coli-derived human FGF-3 protein
Asp28 - Arg212, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
21.1 kDa

Product Datasheets

PRD1206

Reconstitution For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot® directly to the medium for immediate use.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
 • 6 months from date of receipt at room temperature. 
 • 12 months from date of receipt at 2-8 °C as supplied. 
 • 1 month at 2-8 °C under sterile conditions after reconstitution. 
 • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Bioactivity ProDots Recombinant Human FGF-3 Protein Bioactivity View Larger

ProDots® Recombinant Human FGF-3 stimulated cell proliferation of the NR6R-3T3 mouse fibroblast cell line. The ED50 for this effect is 0.02-0.1 μg/mL.

SDS-PAGE ProDots Recombinant Human FGF-3 Protein SDS-PAGE View Larger

1 μg/lane of ProDots® Recombinant Human FGF-3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 21 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-3

Fibroblast growth factor 3 (FGF-3) belongs to the large FGF family which has at least 23 members. All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair. Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated.

The FGF-3 gene, originally designated int-2, was first identified as a proto-oncogene activated in mouse mammary tumors by proviral integration. Amplification of this gene has also been found frequently in human tumors. Human FGF-3 cDNA predicts a 239 aa precursor protein with a 17 aa signal peptide and a 222 aa secreted mature protein with one potential N-linked glycosylation site. Human and mouse FGF-3 share 88% aa sequence identity. The Xenopus and mammalian secreted FGF-3 are processed proteolytically at both the N- and C-terminus. FGF-3 binds with high-affinity to the IIIb isoforms of FGF R1 and FGF R2. FGF-3 also binds the IIIc isoform of FGF R2, but with lower affinity. FGF-3 has been implicated in the induction of inner ear development. Recent studies have suggested that FGF-3 and FGF-8 function synergistically in otic placode induction and during neuronal development to regulate dorsoventral axis formation. During development, the activities of FGF-3 and FGF-8 are regulated negatively by the sprouty family proteins and by sef (similar expression to FGF genes), a transmembrane protein that shares intracellular sequence similarities with the IL-17 receptor.

Long Name
Fibroblast Growth Factor 3
Entrez Gene IDs
2248 (Human)
Alternate Names
FGF3; FGF-3; fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog); fibroblast growth factor 3; HBGF-3; Hst; INT-2; mouse; oncogene INT2

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