ProDots Recombinant Human LIF Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD7734-10
PRD7734-50
PRD7734-01
ProDots Recombinant Human LIF Protein Bioactivity
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ProDots Recombinant Human LIF Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium


Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
Source
E. coli-derived human LIF protein
Pro24 - Phe202
Accession #
N-terminal Sequence
Analysis
Pro24
Predicted Molecular Mass
19.6 kDa
SDS-PAGE

19 kDa, reducing conditions.

Product Datasheets

PRD7734

Reconstitution

For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied.
• 12 months from date of receipt at 2-8 °C as supplied.
• 1 month at 2-8 °C under sterile conditions after reconstitution.
• 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Bioactivity ProDots Recombinant Human LIF Protein Bioactivity View Larger

ProDots® Recombinant Human LIF demostrates cellproliferation activity in the TF-1 human erythroleukemic cell line.The ED50 for this effect is 0.02-0.12 ng/mL.

SDS-PAGE ProDots Recombinant Human LIF Protein SDS-PAGE View Larger

1 μg/lane of ProDots® Recombinant Human LIF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 19 kDa and 17 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIF

Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.

Long Name
Leukemia Inhibitory Factor
Entrez Gene IDs
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names
CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI

FAQs

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Reconstitution Buffers

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