LIF (Leukemia Inhibitory Factor) is a pleiotropic member of the IL-6 family of cytokines. It functions through a heterodimeric receptor complex containing a ligand-binding subunit, LIF R alpha/CD118, and a signal transducing subunit, gp130. gp130 also serves as a subunit of the receptor complexes for Oncostatin M, Cardiotrophin-1, CNTF, IL-6, IL-11, and IL-27. LIF supports uterine implantation of the embryo, pluripotency in embryonic stem cells, and proliferation of progenitor cells. It promotes regulatory T cell and inhibits Th17 cell differentiation, and it is required by the thymic epithelium to support T cell maturation. It promotes motor neuron survival and oligodendrocyte myelination, enhances adrenal production of cortisol and aldosterone, and promotes differentiation of adipocytes and cardiac smooth muscle cells. LIF can also function as an autocrine growth factor in pancreatic cancer and can induce formation of immunosuppressive tumor-associated macrophages.
ProDots Recombinant Human LIF Protein
R&D Systems | Catalog # PRD7734
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
Accession Number
Applications
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
Pro24 - Phe202
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
19 kDa, reducing conditions.
Activity
Scientific Data Images for ProDots Recombinant Human LIF Protein
ProDots Recombinant Human LIF Protein Bioactivity
ProDots®Recombinant Human LIF demostrates cell proliferation activity in the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.02-0.12 ng/mL.
ProDots Recombinant Human LIF Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human LIF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 19 kDa and 17 kDa, respectively.Formulation, Preparation, and Storage
PRD7734
| Reconstitution | For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: LIF
Long Name
Alternate Names
Gene Symbol
UniProt
Additional LIF Products
Product Documents for ProDots Recombinant Human LIF Protein
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human LIF Protein
726224.2
For research use only
Related Research Areas
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