ProDots Recombinant Human Noggin Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD6057-10
PRD6057-50
PRD6057-01
ProDots Recombinant Human Noggin Protein SDS-PAGE
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ProDots Recombinant Human Noggin Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).
Source
Mouse myeloma cell line, NS0-derived human Noggin protein
Gln28 - Cys232
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
23 kDa (monomer)
SDS-PAGE
30-33 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

PRD6057

Reconstitution For a stock solution, reconstitute at 250 μg/mL in sterile PBS. For immediate use, simply roll ProDot® directly into cell culture medium.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied.
• 12 months from date of receipt at 2-8 °C as supplied.
• 1 month at 2-8 °C under sterile conditions after reconstitution.
• 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

SDS-PAGE View Larger

1 μg/lane of ProDots®Recombinant Human Noggin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 30 kDa and 150 kDa, respectively.

Bioactivity View Larger

ProDots®Recombinant Human Noggin inhibits BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50for this effect is typically 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Noggin

Noggin is a secreted homodimeric antagonist of bone morphogenetic proteins (BMPs). It binds BMP-4 with high affinity and BMP-7 with lower affinity. Noggin regulates BMP activity in neural tube formation, somite patterning, cardiomyocyte development, and skeletal joint formation. Mutations of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. Noggin may also regulate the differentiation of embryonic stem cells into dopaminergic neurons.

Entrez Gene IDs
9241 (Human); 18121 (Mouse)
Alternate Names
NOG; Noggin; SYM1; symphalangism 1 (proximal); synostoses (multiple) syndrome 1; SYNS1; SYNS1A

FAQs

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