ProDots Recombinant Human VEGF 165 Protein

Name: ProDots Recombinant Human VEGF 165 Protein
Brand: R&D Systems
SKU: PRD293

R&D Systems | Catalog # PRD293

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Product Documents

Supplied as ready-to-use 5 ug protein balls

Discontinued Product

PRD293 has been discontinued. View all VEGF products.

Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Features and Benefits

Rolls out of bottle for easy cell culture medium preparation
Eliminates time spent aliquoting
One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Source

Sf 21 (baculovirus)

Accession Number

NP_001165097

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all VEGF Proteins and Enzymes »

Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?

ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VEGF protein
Ala27-Arg191

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

19.2 kDa (monomer)

SDS-PAGE

20-22 kDa, reducing conditions
39-42 kDa, non-reducing conditions

Activity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.
The ED₅₀ is 1.0-6.0 ng/mL.

Scientific Data Images for ProDots Recombinant Human VEGF 165 Protein

ProDots Recombinant Human VEGF 165 Protein Bioactivity

ProDots^®VEGF₁₆₅stimulates proliferation in HUVEC human umbilical endothelial cells. The ED₅₀ is 1.0-6.0 ng/mL.

ProDots Recombinant Human VEGF 165 Protein SDS-PAGE

1 μg/mL of ProDots^®Recombinant Human VEGF₁₆₅was resolved by SDS-PAGE with silver staining, under reducing (R) and non-reducing (NR) conditions, showing major bands at 20-22 kDa and 39-42 kDa, respectively. Multiple bands in the gel are due to glycosylation.

Formulation, Preparation, and Storage

PRD293

Reconstitution	For a stock solution, reconstitute at 100 μg/mL in sterile PBS or at 100-500 μg/mL in sterile 4 mM HCl. For immediate use, simply roll ProDot^® directly into cell culture medium.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VEGF

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.