ProDots Recombinant Human VEGF 165 Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD293-10
PRD293-50
PRD293-01
ProDots Recombinant Human VEGF 165 Protein Bioactivity
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ProDots Recombinant Human VEGF 165 Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium


Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 is 1.0-6.0 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VEGF protein
Ala27 - Arg191
Accession #
N-terminal Sequence
Analysis
Ala27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
19.2 kDa (monomer)
SDS-PAGE
20-22 kDa, reducing conditions
39-42 kDa, non-reducing conditions

Product Datasheets

PRD293

Reconstitution For a stock solution, reconstitute at 100 μg/mL in sterile PBS or at 500 μg/mL in sterile 4 mM HCl. For immediate use, simply roll ProDot® directly into cell culture medium.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
•  6 months from date of receipt at room temperature as supplied.
• 12 months from date of receipt at 2-8 °C as supplied.
• 1 month at 2-8 °C under sterile conditions after reconstitution.
• 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Bioactivity ProDots Recombinant Human VEGF 165 Protein Bioactivity View Larger

ProDots® VEGF165 stimulates proliferation in HUVEC human umbilical endothelial cells. The ED50 is 1.0-6.0 ng/mL.

SDS-PAGE ProDots Recombinant Human VEGF 165 Protein SDS-PAGE View Larger

1 μg/mL of ProDots® Recombinant Human VEGF165 was resolved by SDS-PAGE with silver staining, under reducing (R) and non-reducing (NR) conditions, showing major bands at 20-22 kDa and 39-42 kDa, respectively. Multiple bands in the gel are due to glycosylation.   

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

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