Human Profilin 1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-81243

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL
View all Profilin 1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human Profilin 1 in samples. No significant cross-reactivity or interference between Human Profilin 1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.85%

Inter-Assay Precision (Precision between assays) CV% 5.12%

Recovery for Human Profilin 1 ELISA Kit (Colorimetric)

Recovery

87-108%

Linearity

Human Profilin 1 ELISA Kit (Colorimetric)

Scientific Data Images for Human Profilin 1 ELISA Kit (Colorimetric)

Human Profilin 1 ELISA Kit (Colorimetric)

ELISA: Human Profilin 1 ELISA Kit (Colorimetric) [NBP2-81243] -

ELISA: Human Profilin 1 ELISA Kit (Colorimetric) [NBP2-81243] - Standard Curve Reference

Kit Contents for Human Profilin 1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Profilin 1

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Alternate Names

PFN1, PROF1, Profilin I

Gene Symbol

PFN1

Additional Profilin 1 Products

Product Documents for Human Profilin 1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Profilin 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Cancer Biomarkers

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