Progesterone Antibody (6-5E-3F) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-78056

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Radioimmunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6-5E-3F

Format

Azide and BSA Free
View all Progesterone Primary Antibodies »

Product Specifications

Immunogen

Progesterone hemisuccinate conjugated to bovine serum albumin

Localization

Secreted and cytoplasmic

Specificity

This monoclonal antibody is specific for progesterone. It exhibits minimal cross reactivity with related compounds in ELISA. It reacts with Progesterone-11a-HMS-BSA: 100%; 5-beta-Pregnane-3,20-dione: 48%; 5-alpha-Pregnane-3,20-dione: 26.4%; 17-alpha-Hydroxyprogesterone: 2.5%; 20-alpha-Hydroxyprogesterone: 0.04%. Progesterone is a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland. Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Thus, progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Luteinized theca cells of normal ovary secrete progesterone. The determination of progesterone concentrations in the body fluids is of great value for endocrinological investigations in women. This monoclonal antibody may prove useful in identification of ovarian tumors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

314.46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45186).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Progesterone Antibody (6-5E-3F) - Azide and BSA Free

N/A: Progesterone Antibody (6-5E-3F) - Azide and BSA Free [NBP2-78056] - Molecular Structure of Progesterone

Applications for Progesterone Antibody (6-5E-3F) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Radioimmunoassay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: For coating, order Ab without BSA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Progesterone

Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml.

Alternate Names

4-Pregnen-3,20-dione

Additional Progesterone Products

Product Documents for Progesterone Antibody (6-5E-3F) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Progesterone Antibody (6-5E-3F) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Progesterone Antibody (6-5E-3F) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Progesterone Antibody (6-5E-3F) - Azide and BSA Free and earn rewards!

Have you used Progesterone Antibody (6-5E-3F) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies